Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Competitive Inhibition Rat Insulin Elisa Kit , Autoantibody Enzyme Assay Kits 96T

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Wuxi Donglin Sci & Tech Development Co.,Ltd.
[China]

Address
A3-South,100# shuigoutou,renminxi Rd,Wuxi,Jiangsu,PRC. Wuxi Jiangsu
Phone
86-0510-82732223
Contact name
Zhang

Quick Information

  • Brand Name: DLdevelop
  • Place of Origin: China

Description

Competitive Inhibition Rat Insulin Elisa Kit , Autoantibody Enzyme Assay Kits 96T

Short Description:

 

  • FOB Price: Inquiry
  • Min.Order Quantity: 48T
  • Supply Ability: 500 kits/Month
  • Port: Shanghai
  • Payment Terms: T/T in advance

 

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Product Detail

Product Tags

Product name: Rat Insulin Autoantibody (IAA) ELISA Kit
Method: competitive Inhibition
Synonyms:

Anti-Insulin Antibody; Antiinsulin; Insulin Antibodies

Catalog number: DL-IAA-Ra
Detection range: 0.156-10U/mL
Size: 96T
Assay length 1-4.5Hours
Price: inquiry
Quality guarantee period: for 12 months
Introduction
Item Standard Test
Description

The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IAA in rat serum, plasma and other biological fluids.

Conform
Identification Colorimetric Positive
Composition Pre-coated, ready to use 96-well strip plate 1 Conform
Standard (freeze dried) 2
Standard Diluent 120ml
Detection Reagent A 1× 70l
Detection Reagent B 1× 120l
Assay Diluent A 112ml
Assay Diluent B 112ml
TMB Substhumane 19ml
Stop Solution 1 ×6ml
Wash Buffer(30 x concenthumane) 1 ×20ml
Plate sealer for 96 wells 2
Instruction manual 1
Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index.

After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

Recovery

Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-93 86
EDTA plasma(n=5) 80-97 88
heparin plasma(n=5) 90-101 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma(n=5) 88-101% 86-95% 90-102% 80-93%
heparin plasma(n=5) 80-91% 82-90% 95-104% 79-95%
Precision

Intra-assay Precision (Precision within an assay)

 


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