Competitive Inhibition Rat Insulin Elisa Kit , Autoantibody Enzyme Assay Kits 96T
- FOB Price: Inquiry
- Min.Order Quantity: 48T
- Supply Ability: 500 kits/Month
- Port: Shanghai
- Payment Terms: T/T in advance
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||Rat Insulin Autoantibody (IAA) ELISA Kit
Anti-Insulin Antibody; Antiinsulin; Insulin Antibodies
|Quality guarantee period:
||for 12 months
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IAA in rat serum, plasma and other biological fluids.
||Pre-coated, ready to use 96-well strip plate
|Standard (freeze dried)
|Detection Reagent A
|Detection Reagent B
|Assay Diluent A
|Assay Diluent B
|Wash Buffer(30 x concenthumane)
|Plate sealer for 96 wells
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index.
After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
||Recovery range (%)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay)