Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Chemokine Mouse Elisa Kit Motif Receptor Minimum Detectable Dose Less Than 0.061ng/Ml

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Wuxi Donglin Sci & Tech Development Co.,Ltd.
[China]

Address
A3-South,100# shuigoutou,renminxi Rd,Wuxi,Jiangsu,PRC. Wuxi Jiangsu
Phone
86-0510-82732223
Contact name
Zhang

Quick Information

  • Brand Name: DLdevelop
  • Place of Origin: China

Description

Chemokine Mouse Elisa Kit Motif Receptor Minimum Detectable Dose Less Than 0.061ng/Ml

Product Detail

Product Tags

1. Overview

Other names:CD192; CC-CKR2; CCR2A; C-C CKR-2; CCR2B; CKR2-A; CKR2-B; CMKBR2; MCP1-R; Monocyte chemoattractant protein 1 receptor

Function: Receptor for the MCP-1 (JE), MCP-3 (FIC) and MCP-5 chemokines. Transduces a signal by increasing the intracellular calcium ions level.

Sequence:

MEDNNMLPQF IHGILSTSHS LFTRSIQELD EGATTPYDYD DGEPCHKTSV

KQIGAWILPP LYSLVFIFGF VGNMLVIIIL IGCKKLKSMT DIYLLNLAIS

DLLFLLTLPF WAHYAANEWV FGNIMCKVFT GLYHIGYFGG IFFIILLTID

RYLAIVHAVF ALKARTVTFG VITSVVTWVV AVFASLPGII FTKSKQDDHH

YTCGPYFTQL WKNFQTIMRN ILSLILPLLV MVICYSGILH TLFRCRNEKK

RHRAVRLIFA IMIVYFLFWT PYNIVLFLTT FQESLGMSNC VIDKHLDQAM

QVTETLGMTH CCINPVIYAF VGEKFRRYLS IFFRKHIAKR LCKQCPVFYR

ETADRVSSTF TPSTGEQEVS VGL

2. Features

DETECTION RANGE

0.156-10ng/mL. The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL.

SENSITIVITY

The minimum detectable dose of CCR2 is typically less than 0.061ng/mL.

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

SPECIFICITY

This assay has high sensitivity and excellent specificity for detection of CCR2.

No significant cross-reactivity or interference between CCR2 and analogues was observed.

Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between CCR2 and all analogues, therefore, cross reactivity may still exist.

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCR2 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCR2 were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

STABILITY

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.

Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

ASSAY PROCEDURE SUMMARY

1. Prepare all reagents, samples and standards;

2. Add 100L standard or sample to each well. Incubate 2 hours at 37oC;

3. Aspirate and add 100L prepared Detection Reagent A. Incubate 1 hour at 37oC;

4. Aspirate and wash 3 times;

5. Add 100L prepared Detection Reagent B. Incubate 1 hour at 37oC;

6. Aspirate and wash 5 times;

7. Add 90L Substrate Solution. Incubate 15-25 minutes at 37oC;

8. Add 50L Stop Solution. Read at 450nm immediately.

IMPORTANT NOTES

1. Limited by the current conditions and scientific technology, it is impossible to conduct comprehensive identification and analysis tests on the raw materials provided by suppliers. As a result, it is possible there are some qualitative and/or technical risks.

 


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